Detection of dna specific sequences of spongospora. Detection in the soil and on tubers is based on the presence of sporosori, the resting spores of the pathogen. Seedlings of the plants were grown in nutrient solutions and inoculated with spore balls ofs. Monitoring spongospora subterranea development in potato. Pdf improved diagnosis of powdery scab spongospora. Epidemiology and control of spongospora subterranea f. The roots were microscopically examined for the presence of plasmodia or zoosporangia 14 days after inoculation. Though this disease can reduce yield somewhat, the true economic effect is due to the unmarketable, scabbed appearance figure 1 as well as rejection of tubers as seed to prevent. Detection and quantification of spongospora subterranea f. During 19941996 surveys were performed in the netherlands on tubers with symptoms resembling common scab.
Field evaluation of potato genotypes for resistance to. Egan, department of environmental resource management, national university of ireland, belfield, dublin 4, ireland introduction the plasmodiophorid spongospora subterranea f. Sintomas y signos producidos por spongospora subterranea f. Its success is attributed to the characteristics of its pathogen and features of its life cycle. Taller diagnostico spongospora subterranea en papa. Investigating factors affecting infection of potato by spongospora subterranea alison lees, louise sullivan and jennie brierley plant pathology, scri, invergowrie, dundee, dd2 5da, uk conclusions and future work dna of s. Inspections are performed on unwashed samples, incidentally supported by microscopic examination. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. It is caused by the cercozoan spongospora subterranea f. These spores can be detected through bioassays, pcr, and antibody methods.
Iftikhar et al characterization of spongospora subterranea f sp subterranea root hair and epidermal cells of roots under a stereomicroscope with 20fold magnificatiarn rating of root infection done on 04 scale of marz 1989, vvhsre 0 no sporangia, 1 only a fevv sporangia, several roots with sporangia, 3 sporangia regularly. Pt303 epidemiology and control of powdery scab of potatoes. Powdery scab effect on the potato solanum tuberosum ssp. Powdery scab affects most potato production zones in the world. Global genetics and invasion history of the potato powdery. Tecaphora solani ralstonia solanacearum pectobacterium carotovorum p. Marcela villegas ochoa2 y elena paola gonzalez jaimes3 resumen. They are sent to a computer by website operators or third parties. Spongospora subterranea sponsuoverview eppo global database. Improved diagnosis of powdery scab spongospora subterranea. A new distribution map is provided for spongospora subterranea wallr. Diacol capiro differential distrubution of candidadate biocontrol bacteria against spongospora subterranea in potato plants solanum tuberosum cv. Transactions of the british mycological society, 41. Resistance of potato to powdery scab spongospora subterranea under andean field conditions.
Production of cystosori of spongospora subterranea walk. Hairy nightshade is an alternative host of spongospora. Parbery school of agriculture and forestry, university of melbourne, parkville 3052, australia evidence that potato tubers are susceptible to infection by spongospora subterranea only during lenticel formation and proliferation is presented and the capacity of apparently. Class project for pp 728, soilborne plant pathogens, fall 2012 introduction. Pdf detection and quantification of spongospora subterranea.
Investigating factors affecting infection of potato by. Three diseases of potato caused by spongospora subterranea. Duncan, scottish crop research institute, invergowrie, dundee dd2 5da, scotland, uk. Symptoms of powdery scab include small lesions in the early stages of the disease, progressing to raised pustules containing a powdery mass. Epidemiology of spongospora subterranea, the cause of. Spongospora subterranea is the causal agent of powdery scab on potato. This study was performed to determine the host range ofspongospora subterranea f. Infection of potato by spongospora subterranea sciencedirect. Project report epidemiology, autecology and control of spongospora subterranea, cause of potato powdery scab ref. Based on visual inspection, discrimination between common scab streptomyces spp. Field evaluation of potato genotypes for resistance to powdery scab on tubers and root gall formation caused by spongospora subterranea francisco g. Effect of environmental vectors on infection of potato by spongospora subterranea f. Autrefois classe parmi les champignons, cet organisme est aujourdhui rattache aux protozoaires division rhizaria, embranchement cercozoa. Global genetic characterisation of the powdery scab pathogen introduction powdery scab is one of the most serious diseases of potatoes wherever intensive potato cropping is carried out.
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